Scientific part of my journey

Reading my posts, it looks like I am more enjoying the cultural part and almost forgot the main reason I crossed the Atlantic with the Fulbright wings.

The first month in the lab was more a warming up. Where is my desk? Where is the cell culture rooms? How do they run it? How different is it? So, many microscopes – am I capable of imaging? And so on and so forth…

My typical day starts at 8-8.30 am and finishes once all is done. It may be 6pm or 10pm. Once the experiment is set up, I have to monitor cells every 24 hours for 5-7 days with no weekends or days off. The monitoring includes imaging. Lots of imaging. Every condition has 20-30 single cells to follow up. Each cell gets its own GPS tag manually to be able to image exactly the same cell as it grows and becomes a group of hundreds by multiplication. For example, I am running 8 different cell lines in 3 experimental conditions. So, 20-30 cells per all 24 combinations give us 480-720 individual cells to follow up. The imaging takes ~5 hours every day. After 5 days, I will have 2400 – 3600 pics of cells to analyse. It will be fun! I may need lots of Guinness to fly through that numbers.

Tagging cells. The left arrow points to a group of neuroblastoma cells. The arrow in the middle point to the same cells, but this image allows you to see the actual number of the cells. This group has 8 cells. The right arrow points to individual GPS tags for each cell

At the next step, I will select some of the conditions for video recording to trace cell fate from a single neuroblastoma cell to a metastatic niche consisting of hundreds of them. This video will show me how it all happens minute after minute.

Is not it exciting? I am thrilled!

 

Every child deserves a happy childhood

Three girls fountain in Mainz Germany

Last year I have selected this photo of a lovely fountain capturing 3 girls under umbrellas (Drei-Mädchen-Brunnen) in Ballplatz Mainz in support of #ChildhoodCancerAwarnessMonth. This fountain was built between two Catholic girl’s schools symbolising the separate education and a happy childhood. It is charming on its own. And I’ve select it again.
Every child deserves a happy childhood. Raising awareness about childhood cancer we help to make the dreams of children with cancer come true. Dreams for a happy childhood, better treatment, better quality of life full of love ahead through better funding of childhood cancer research and access to innovative treatments.

September is Childhood Cancer Awareness Month!

Today marks the start of Childhood Cancer Awareness Month.

Three girls fountain in Mainz Germany 

The cause of childhood cancers is believed to be due to faulty genes in stem cells that give rise to nerves, skin, blood and other body tissues. For some unknown reasons, the faulty genes can sit quiet and show their ‘bad’ character after birth and programme the cells into cancer cells.
So, there is no evidence that links lifestyle or environmental risk factors to the development of childhood cancer, which is opposite to many adult’s cancers.

Every 100th cancer patient is a child. Cancer is the 2nd most common cause of death among children after accidents.

Children are not little adults and so their cancer. Some childhood cancers have a good outlook and successful protocol of treatments. However, some of the cancers do not respond to the known drugs, or if respond cancer cells find the way to develop resistance and come back being more aggressive. Among theme are some forms of brain tumours, neuroblastoma and sarcomas; cancers developing in certain age groups and/or located within certain sites in the body, along with acute myeloid leukaemia (blood cancer). Children with a rare brain cancer – diffuse intrinsic pontine glioma survive less than 1 year from diagnosis. Children with soft tissue tumours have 5-year survival rates ranging from 64% (rhabdomyosarcoma) to 72% (Ewing sarcoma). Less than50% of children with the aggressive form of neuroblastoma will live beyond 5 years with current treatment strategies.

For majority of children who do survive cancer, the battle is never over. Over 60% of long‐term childhood cancer survivors have a chronic illness as a consequence of the treatment; over 25% have a severe or life-threatening illness.

The most common types of childhood cancer are:

  • Leukaemia and lymphoma (blood cancers)
  • Brain and other central nervous system tumours
  • Muscle cancer (rhabdomyosarcoma)
  • Kidney cancer (Wilms tumour)
  • Neuroblastoma (tumour of the non-central nervous system)
  • Bone cancer (osteosarcoma)
  • Testicular and ovarian tumours (gonadal germ cell tumours)

Please see a short video The Childhood Cancer Ripple Effect created by St. Baldrick’s Foundation.

Saying Good Bye to Our Summer Students

When I look back at the end of July, I am always surprised how quickly 8 summer weeks passed by. Summer students usually come very shy and uncertain and then they are flying through many complicated research terms and techniques. We help them to learn and they pay back by fantastic enthusiasm, commitment, and hard work. This summer was the same!

One of our experiences was donut’s tasting. We tasted donuts from Boston Donuts, the Rolling Donut, Boomerang Donuts and Krust Bakery. Many shapes, textures, and tastes. Krust Bakery did our favourites. 🙂

Saying Good bye to our summer students

 

Research Summer School 2018

Another year, another Research Summer School students. We are hosting 4 students (Jessica, Dawn, Dola, and Jeff) this year. Some of them will be medical doctors, another will do research after the graduation. For them, the 8-weeks lab placement is a window into the reality of the everyday science. How cancer cells look? How do they grow? Where do we store them? How do we know that we have identified a new drug or a new target to study further? Do researchers have a sense of humour? Do they like donuts?

Why do they wear these astronaut helmets?

We have already said Good Bye to Jessica. Dola and Dawn’s projects are coming to an end this week, while Jeff is staying till the end of August.

A new, three-dimensional approach to cancer research

Appeared in today’s Irish Times. Lovely crafted by Dr. Vanesa Martinez

Although the discovery could be applicable in principle to any a solid tumour, Dr Piskareva’s target is neuroblastoma, a relatively common child cancer which affects a specific type of nerve cells in unborn children. “It’s quite aggressive and unfortunately there are many children who have metastasis when they are diagnosed, and this is the most challenging group to treat.”

Irish Times, 31 May 2018

https://www.irishtimes.com/news/science/a-new-three-dimensional-approach-to-cancer-research-1.3505347

Groundwork

Though the official announcement is scheduled for the first week of June, the groundwork is on. Lots of reading and planning for the trip to Johns Hopkins later this year. One of the first is the book by Rebecca Skloot ‘The Immortal Life Of  Henrietta Lacks”. The famous HeLa cells were generated by researchers at JH. The story is a fascinating journey for biomedical scientists and a tragedy for the Lacks family.

How is it feeling?

The fact of being shortlisted is very encouraging. It means that my research proposal and the career achievements fit the merit of this award.  No doubt it was fantastic experience overall, not often the shortlisted candidates have an opportunity to speak for themselves.

How is it feeling after the interview?… Well, I do not have a firm answer… It is a big difference when you explain yourself in written and spoken forms… No chance to edit your real-time talk… How many times have that 30 minutes played back and re-run in my head? I lost the count… Did I bring the point across? Did I do things in right time and at a right pace? Should I have structured the answer differently? Each re-run brings new ways to answer the same questions, indeed, in a better and more concise way…   Having the mind that is constantly analysing the situation is not helpful.

Think, the competition was very tough, and only 1 in 10 made to the 2 days interview for the Fulbright Award (maybe the ratio even higher). Twelve candidates were interviewed yesterday and the same numbers are to be today.  What are the chances to get to the final? I have to wait until March… and meanwhile, keep applying for grants and doing something meaningful.

My next stop is at the Irish Cancer Society this Thursday to film a short video about my research and neuroblastoma challenges. The video should be available for the International Childhood Cancer Research Day on February 15th.

The First Big Challenge in New Year

The first month of the new year and the first challenge. Monday is the big day for me. In the essence, my grant application was shortlisted for the interview where I have to face the challenge and prove that I worth it.

Anyone for a challenge?

The proposed application seeks to go to the US lab and gain an expertise in an interdisciplinary methodology to monitor and capture the dynamic of cancer spread (metastasis) in real time. This experimental approach would accelerate our understanding of neuroblastoma metastasis which is one of the reasons for failure in the treatment of neuroblastoma. If we know how neuroblastoma cells spread, then we can find the weaknesses in the process and create new drugs or use existing to target it.

I feel that sharing my worries with you makes me stronger. I am looking forward to this challenge with my head up and hope to feel your support at this crucial moment.