Blog about neuroblastoma research – Page 17 – My WordPress Blog is about neuroblastoma biology and Cancer Bioengineering Group

Scientific part of my journey

Reading my posts, it looks like I am more enjoying the cultural part and almost forgot the main reason I crossed the Atlantic with the Fulbright wings.

The first month in the lab was more a warming up. Where is my desk? Where is the cell culture rooms? How do they run it? How different is it? So, many microscopes – am I capable of imaging? And so on and so forth…

My typical day starts at 8-8.30 am and finishes once all is done. It may be 6pm or 10pm. Once the experiment is set up, I have to monitor cells every 24 hours for 5-7 days with no weekends or days off. The monitoring includes imaging. Lots of imaging. Every condition has 20-30 single cells to follow up. Each cell gets its own GPS tag manually to be able to image exactly the same cell as it grows and becomes a group of hundreds by multiplication. For example, I am running 8 different cell lines in 3 experimental conditions. So, 20-30 cells per all 24 combinations give us 480-720 individual cells to follow up. The imaging takes ~5 hours every day. After 5 days, I will have 2400 – 3600 pics of cells to analyse. It will be fun! I may need lots of Guinness to fly through that numbers.

Tagging cells. The left arrow points to a group of neuroblastoma cells. The arrow in the middle point to the same cells, but this image allows you to see the actual number of the cells. This group has 8 cells. The right arrow points to individual GPS tags for each cell

At the next step, I will select some of the conditions for video recording to trace cell fate from a single neuroblastoma cell to a metastatic niche consisting of hundreds of them. This video will show me how it all happens minute after minute.

Is not it exciting? I am thrilled!

September Highlights

I pass this traffic light on my way to work every morning. It has all mentioned in the text: the street label, and the additional sign regarding ‘red’ light rules.

The first month flew in a flash. Vibrant and exciting. What did surprise me? Well… No public transport in Baltimore as we got used to in Dublin. Driver friendly environment. I like street indications at the junctions. Helps a lot in navigation. Clear and straight to the point. You can drive anywhere, you find a parking spot within 1-2 minutes, though the fee can be very high if in town. Around Johns Hopkins Hospital street parking is just 1 USD per hour, though limited to max 2 hours. You can turn on ‘red’ if no danger posses. As it always happens some drivers read this rule on demand and turn on ‘red’ even the additional road sign prohibits it. Many jammed/bumped cars in use.

Ok. Baseball. I and some man from my host family went to see a match of the local team Baltimore Orioles vs Chicago White Sox. I like their positive team colours – orange. It was sweet to get a free orange hoodie at the game and eat a standard size ice cream cone – enough to feed 2-3 people. Their stadium is a big museum of Orioles success. Nice to rambler around. The team is not doing well in the current season. They lost 0:2. Many players are traded in and out. Did I gain all insights of the game? Hmmm… But, wish the team and the fans best of luck in the next season!

What about the American Church? Yep, I did it, too! I attended the worship service in Glen Mar Church – a United Methodist congregation. Wikipedia says that the United Methodist Church (UMC) is a mainline Protestant denomination and a major part of Methodism. Did not hear about it much, but I am staying with the family who is not just active members of the UMC, but the head of the family is a recently retired pastor. So, I went there for his last service. It is very different to Catholic or Orthodox and the feeling that you get entirely different. Pastor and church members interact actively and not passively. It feels more like a family or person consulting and support environment rather than a dogmatic lectureship.

However, my main highlight is NASA. And it deserves a separate story.

Short & Sweet Trip to NY

This is how my journey began. On Tuesday, I received an invite from the Children’s Medical Research Foundation to attend the Annual Summer Gala Dinner at the Water Club in Manhattan on Thursday the same week. Are two days enough to plan your trip? Perhaps it depends on many things, I had no reservations. The only one uncertainty was car parking logistics.

Those, who are familiar with Baltimore history and current life, know that your car is your Castle. After the Internet search and chats with my host family, the plan was to get a bus to NY which provides a designated car park.

Three hours on the bus flew in a flash. Wide roads with trees on both sides did remind me of some motorways in Russia. Gigantic tolls – almost 12 lanes in both directions. An impressive tunnel under the Hudson River connects the mainland with Manhatten. One can see a borderline dividing the tunnel into New Jersey and New York parts on about halfway.

What was my first impression of NY once I got off the bus? Many snapshots instantly jumped in linking with Hollywood movies that pictured NY. Mostly from ‘Sex and the City’. A traffic jam here and there. Crazy Taxi and car drivers. Brainless pedestrians. Everyone on the run. Madness. I did enjoy it as a tourist. Would I cope with it on everyday bases? A very big question!

Unfortunately, thanks to the hurricane Florence, not much sightseeing was on offer. All tall buildings were hidden with clouds. Tourists were queuing for hop-on-off.

I had 2 hours before the bus to Baltimore. My choice was Times Square. A classical picture – huge screens are talking to you offering pleasure and entertainment. Come in, relax and enjoy!

Strolling around Cafe shops, theaters, food vans. Looking at tourists and locals. Feeling and absorbing… Short, but sweet.

Christmas never ends at Times Square!

Many things to do next time…

Every child deserves a happy childhood

Last year I have selected this photo of a lovely fountain capturing 3 girls under umbrellas (Drei-Mädchen-Brunnen) in Ballplatz Mainz in support of #ChildhoodCancerAwarnessMonth. This fountain was built between two Catholic girl’s schools symbolising the separate education and a happy childhood. It is charming on its own. And I’ve select it again.
Every child deserves a happy childhood. Raising awareness about childhood cancer we help to make the dreams of children with cancer come true. Dreams for a happy childhood, better treatment, better quality of life full of love ahead through better funding of childhood cancer research and access to innovative treatments.

Labour Day BBQ

Ok. This Monday is Labour Day – a public holiday celebrated on the 1st Monday in September in the US. According to the US Department of Labor, this holiday marks “a creation of the Labor movement and is dedicated to the social and economic achievements of American workers”. In contrast in Europe and Russia, it is celebrated on May 1st and known as May Day or International Workers’ Day and may or may not be a public holiday.

It is also the unofficial end of summer when many people arrange family gatherings or holidays. So, did our lab. We were all invited to our boss’ house to have a BBQ and chit-chat. As you may expect almost everyone in the lab has a multicultural background which is very proud of. You are in America, babe! I am not an exception. A proud Irish-Russian.

Irish black and white pudding, red and white cheddar and homebaked soda bread were among top 10 favourites

Everyone took advantage of and benefited from that mix. We had Mexican, Argentinian, French, Irish, Jewish, Ethiopian and American Indiana, Idaho, Florida, Maryland bites. Juicy steaks (raw, medium and well done) and burgers grilled by the host Andy were delicious. Have to admit that meat was tastier than I used to buy in Ireland. Should probably look for a new butcher when I come back!

What did surprise me the most? I have been thinking about it on the way home… None of 15 guests did check their mobile or take a pic of food/selfie during that time! Though everyone had this thing in the pocket. We were chatting and laughing. Maybe it is just that people… Fantastic company and a great day out.

September is Childhood Cancer Awareness Month!

Today marks the start of Childhood Cancer Awareness Month.

The cause of childhood cancers is believed to be due to faulty genes in stem cells that give rise to nerves, skin, blood and other body tissues. For some unknown reasons, the faulty genes can sit quiet and show their ‘bad’ character after birth and programme the cells into cancer cells.
So, there is no evidence that links lifestyle or environmental risk factors to the development of childhood cancer, which is opposite to many adult’s cancers.

Every 100th cancer patient is a child. Cancer is the 2nd most common cause of death among children after accidents.

Children are not little adults and so their cancer. Some childhood cancers have a good outlook and successful protocol of treatments. However, some of the cancers do not respond to the known drugs, or if respond cancer cells find the way to develop resistance and come back being more aggressive. Among theme are some forms of brain tumours, neuroblastoma and sarcomas; cancers developing in certain age groups and/or located within certain sites in the body, along with acute myeloid leukaemia (blood cancer). Children with a rare brain cancer – diffuse intrinsic pontine glioma survive less than 1 year from diagnosis. Children with soft tissue tumours have 5-year survival rates ranging from 64% (rhabdomyosarcoma) to 72% (Ewing sarcoma). Less than50% of children with the aggressive form of neuroblastoma will live beyond 5 years with current treatment strategies.

For majority of children who do survive cancer, the battle is never over. Over 60% of long‐term childhood cancer survivors have a chronic illness as a consequence of the treatment; over 25% have a severe or life-threatening illness.

The most common types of childhood cancer are:

Leukaemia and lymphoma (blood cancers)
Brain and other central nervous system tumours
Muscle cancer (rhabdomyosarcoma)
Kidney cancer (Wilms tumour)
Neuroblastoma (tumour of the non-central nervous system)
Bone cancer (osteosarcoma)
Testicular and ovarian tumours (gonadal germ cell tumours)

Please see a short video The Childhood Cancer Ripple Effect created by St. Baldrick’s Foundation.

And finally, the Fulbright journey has started

Air Canada was my first bridge to connect me and Baltimore. A new luxury plane with great service brought me over Atlantic to Montreal in 7 hours. Watched many films and TV movies starting with ‘ Battle of the Sexes’ and finishing with re-running some episodes of ‘Young Sheldon’. Food was excellent, fast service, great taste. The US Customs met me with two state flags flanking a copy of the Liberty statue at the Canadian Airport, so had no need to do anything at the final destination. My 2nd leg was way shorter – just 1.30hrs!

At BWI a lovely American family picked me and my bag up and brought to their sister’s house – my American home for the next 4 months. It is promised to be a true cultural experience. How I found them? It is a story for a separate post to follow.

My American house is in Baltimore outskirts and in 30 min drive from door to door. My first drive to work was a break of all statistical predictions about tyre’s punctures. One of the tyres went flat almost on the half-way in probably not a very safe neighbourhood. With no cell phone, no roaming and no a spare ‘donut’!

What happened next? ‘Emergency lights’-ON, pulling my hand out to stop anyone with a cell phone. After a couple of dozens passing by cars, one pulled down, a nice couple of doctors offered me their help. Phoned to my host family and explained the situation. “Stay in the car with locked doors, we are on the way”. While I was waiting for them a nice man from the Afro-American neighborhood tried to help me out. “No, no… thank you… I am fine… Help is on the way…” in a sort of fear I replied.

Next, both I and Rod (the head of the family) were looking for a spare ‘donut’ and tools to lift up the car. Nothing. The same nice man came again offering his help. We did not resist. He pulled his brand new shiny Lincoln Continental and brought all you can dream of in my situation. A jack to lift the car, an electric screwdriver to get the tyre off… His name is Melvin. I and Rod run into the nearest garage to fix the tyre, while Melvin was looking after my car.

Forty-five minutes later, I continued my journey and arrived 2 hours later than planned. Rush to hunt the ID and car parking. A long queue of new commenced stuff needed exactly the same and disappeared in 25 min. Interestingly, I got the spot in 5 min for my use during the next 4 months as all the rest from the queue. Imagine, to get something like this in Dublin Centre area. Now, my car is parked in 5 min walk from the building I work in. A nice end of the first day!

Facebook Collection

I have a nice collection of pictures related to our lab activities or research, not all of them were posted here. Hope, that Facebook could provide an additional nice platform to store and share them. I am grouping them by theme in an album and link with a relevant blog post.

Let see how it would work!

Saying Good Bye to Our Summer Students

When I look back at the end of July, I am always surprised how quickly 8 summer weeks passed by. Summer students usually come very shy and uncertain and then they are flying through many complicated research terms and techniques. We help them to learn and they pay back by fantastic enthusiasm, commitment, and hard work. This summer was the same!

One of our experiences was donut’s tasting. We tasted donuts from Boston Donuts, the Rolling Donut, Boomerang Donuts and Krust Bakery. Many shapes, textures, and tastes. Krust Bakery did our favourites. 🙂

Research Summer School 2018

Another year, another Research Summer School students. We are hosting 4 students (Jessica, Dawn, Dola, and Jeff) this year. Some of them will be medical doctors, another will do research after the graduation. For them, the 8-weeks lab placement is a window into the reality of the everyday science. How cancer cells look? How do they grow? Where do we store them? How do we know that we have identified a new drug or a new target to study further? Do researchers have a sense of humour? Do they like donuts?

Why do they wear these astronaut helmets?

We have already said Good Bye to Jessica. Dola and Dawn’s projects are coming to an end this week, while Jeff is staying till the end of August.